Principles of gene manipulation and genomics / S.B. Primrose and R.M. Twyman.Material type: TextPublication details: Malden, MA ; Oxford : Blackwell Pub., 2006Edition: 7th edDescription: xxii, 644 p. : ill. ; 28 cmISBN: 1405135441 (pbk. : alk. paper); 9781405135443Subject(s): Genetic engineering | Genomics | Gene mapping | Nucleotide sequence | Genetic Engineering | Base Sequence | Chromosome Mapping | DNA, Recombinant | GenomicsDDC classification: 660.65 PRP LOC classification: QH442 | .O42 2006NLM classification: 2006 H-690 | QH 442Online resources: Table of contents
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Rev. ed. of: Principles of gene manipulation. 6th ed. 2001 and Principles of genome analysis and genomics / Sandy B. Primrose, Richard M. Twyman. 3rd ed. 2003.
Includes bibliographical references (p. -626) and index.
Gene manipulation in the post-genomics era -- Basic techniques -- Cutting and joining DNA molecules -- Basic biology of plasmid and phage vectors -- Cosmids, phasmids, and other advanced vectors -- Gene cloning strategies -- Sequencing genes and short stretches of DNA -- Changing genes : site-directed mutagenesis and protein engineering -- Bioinformatics -- Cloning in bacteria other than Escherichia coli -- Cloning in Saccharomyces cerevisiaea and other fungi -- Gene transfer to animal cells -- Genetic manipulation of animals -- Gene transfer to plants -- Advanced transgenic technology -- The organization and structure of genomes -- Mapping and sequencing genomes -- Comparative genomics -- Large-scale mutagenesis and interference -- Analysis of the transcriptome -- Proteomics I : expression analysis and characterization of proteins -- Proteomics II : analysis of protein structures -- Proteomics III : protein interactions -- Metabolomics and global biochemical networks -- Applications of genomics : understanding the basis of polygenic disorders and identifying quantitative trait loci -- Applications of recombinant DNA technology.